Anti-NCBP1/Cbp80 Antibody

Product Name

Anti-NCBP1/Cbp80 Antibody

View all NCBP1 Antibodies

SKU/Catalog Number

A07424

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-NCBP1/Cbp80 Antibody (Catalog # A07424). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NCBP1/Cbp80 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07424)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human NCBP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07424 is reactive to NCBP1 in Human, Mouse, Rat

Applications

A07424 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

91.839kDa

Background of NCBP1

Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.

Izaurralde E., Cell 78:657-668(1994).
Kataoka N., Nucleic Acids Res. 22:3861-3865(1994).
Humphray S.J., Nature 429:369-374(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from HT-29 cells and LOVO cells, using NCBP1 antibody A07424.

Gene/Protein Information For NCBP1 (Source: Uniprot.org, NCBI)

Alternative Names

CBP80nuclear cap binding protein subunit 1, 80kD; NCBP 80 kDa subunit; NCBPMGC2087; nuclear cap binding protein subunit 1, 80kDa; nuclear cap-binding protein subunit 1,80 kDa nuclear cap-binding protein; Sto1 NCBP1 CBP80, NCBP, Sto1 nuclear cap binding protein subunit 1 nuclear cap-binding protein subunit 1|80 kDa nuclear cap-binding protein|NCBP 80 kDa subunit|nuclear cap binding protein subunit 1, 80kDa

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